anti col1 Search Results


90
ImmunoGlobe Antikoerpertechnik anti–type i collagen cleavage site antibody #0217-050
Anti–Type I Collagen Cleavage Site Antibody #0217 050, supplied by ImmunoGlobe Antikoerpertechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ZenBio anti-col-1
Anti Col 1, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Abnova anti-human col1 monoclonal mouse antibody clone i-8h5
Anti Human Col1 Monoclonal Mouse Antibody Clone I 8h5, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quartett GmbH antibody anti-col1
Antibody Anti Col1, supplied by Quartett GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ImmunoGlobe Antikoerpertechnik rabbit anti-collagen type cleavage site antibody (col1¾c short
Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 <t>mg/ml</t> <t>collagen</t> after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, <t>COL1</t> ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.
Rabbit Anti Collagen Type Cleavage Site Antibody (Col1¾c Short, supplied by ImmunoGlobe Antikoerpertechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation human pro collagen i alpha 1 antibody
Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 <t>mg/ml</t> <t>collagen</t> after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, <t>COL1</t> ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.
Human Pro Collagen I Alpha 1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bioss collagen i monoclonal antibody
Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 <t>mg/ml</t> <t>collagen</t> after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, <t>COL1</t> ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.
Collagen I Monoclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bioss collagen 1 polyclonal antibody
Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 <t>mg/ml</t> <t>collagen</t> after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, <t>COL1</t> ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.
Collagen 1 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation collagen i alpha 1 antibody (col-1)
Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 <t>mg/ml</t> <t>collagen</t> after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, <t>COL1</t> ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.
Collagen I Alpha 1 Antibody (Col 1), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+col1/Collagen+I+alpha+1+Antibody+(COL-1)/custom%40nb600-450%4040730191
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86
Servicebio Inc anti col
Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 <t>mg/ml</t> <t>collagen</t> after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, <t>COL1</t> ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.
Anti Col, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bioss pro-collagen 1 polyclonal antibody
Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 <t>mg/ml</t> <t>collagen</t> after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, <t>COL1</t> ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.
Pro Collagen 1 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Enzo Biochem anti-human col-1
Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 <t>mg/ml</t> <t>collagen</t> after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, <t>COL1</t> ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.
Anti Human Col 1, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 mg/ml collagen after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, COL1 ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.

Journal: bioRxiv

Article Title: Extracellular matrix guidance determines proteolytic and non-proteolytic cancer cell patterning

doi: 10.1101/2022.03.16.484647

Figure Lengend Snippet: Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 mg/ml collagen after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, COL1 ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.

Article Snippet: Affinity purified rabbit anti-collagen type I cleavage site antibody (Col1¾C short , Immunoglobe), Alexa-488-conjugated secondary pre-absorbed goat anti-rabbit IgG (Invitrogen), DAPI (Sigma) and Alexa-568-conjugated phalloidin (Invitrogen) were used.

Techniques: Migration, Activity Assay, Derivative Assay, In Silico, In Vitro, MANN-WHITNEY