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Image Search Results
Journal: bioRxiv
Article Title: Extracellular matrix guidance determines proteolytic and non-proteolytic cancer cell patterning
doi: 10.1101/2022.03.16.484647
Figure Lengend Snippet: Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 mg/ml collagen after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, COL1 ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.
Article Snippet:
Techniques: Migration, Activity Assay, Derivative Assay, In Silico, In Vitro, MANN-WHITNEY